EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Re evaluation of the effects of glucose 6 phosphate and malate on the catalytic properties of phosphoenolpyruvate carboxylase from cynodon dactylon under physiological assay conditions



Re evaluation of the effects of glucose 6 phosphate and malate on the catalytic properties of phosphoenolpyruvate carboxylase from cynodon dactylon under physiological assay conditions



Australian Journal of Plant Physiology 17(4): 407-412



Some catalytic and regulatory properties of the day- and night-form of phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Cynodon dactylon (L.) Pers. leaves were re-evaluated in the presence of high concentrations of glycerol in the assay medium. This cosolute, known to be preferentially excluded from the hydration sphere of some proteins, apparently promotes aggregation, through an increase of the enzyme concentration in a fraction of the total volume. In the presence of this cosolute in the assay medium: (a) glucose-6-phosphate does not activate the enzyme, (b) inhibition of the Vmax by malate is increased, particularly with the night-form of phosphoenolpyruvate carboxylase, whereas the day-form is more responsive at the Km levels, (c) the light activation, detected as an increase in the apparent affinity of the substrate phosphoenolpyruvate is maintained under the new assay conditions.

(PDF emailed within 1 workday: $29.90)

Accession: 007722998

Download citation: RISBibTeXText



Related references

Effects of phosphate on the activity, stability and regulatory properties of phosphoenolpyruvate carboxylase from the C-4 plant Cynodon dactylon. Photosynthetica (Prague) 33(2): 189-197, 1997

Modulation by bicarbonate of catalytic and regulatory properties of C4 phosphoenolpyruvate carboxylase from Amaranthus hypochondriacus: Desensitization to malate and glucose 6-phosphate and sensitization to Mg2+. Plant & Cell Physiology 39(12): 1294-1298, 1998

Phosphate and sulfate activate the phosphoenolpyruvate carboxylase from the C-4 plant Cynodon dactylon L. Botanica Acta 110(4): 309-313, 1997

Organic cosolutes increase the catalytic efficiency of phosphoenolpyruvate carboxylase from cynodon dactylon l. pers. apparently through self association of the enzymic protein. Australian Journal of Plant Physiology 15(5): 621-632, 1988

The Interactive Effects of pH, L-Malate, and Glucose-6-Phosphate on Guard-Cell Phosphoenolpyruvate Carboxylase. Plant Physiology 103(4): 1189-1194, 1993

Trehalose, an extreme temperature protector of phosphoenolpyruvate carboxylase from the C4-plant Cynodon dactylon. Phytochemistry (Oxford) 46(8): 1331-1334, 1997

A study of the in-vitro regulation of phosphoenolpyruvate carboxylase from the epidermis of Commelina communis by malate and glucose-6-phosphate. Planta 155(5): 416-422, 1982

Effects of sulfite on phosphoenolpyruvate carboxylase and nad phosphate dependent malate dehydrogenase in epidermal peels in two cultivars of pea. Physiologia Plantarum 79(3): 491-496, 1990

Effects of sulfite on phosphoenolpyruvate carboxylase and nicotinamide adenine dinucleotide phosphate-dependent malate dehydrogenase in epidermal peels in two cultivars of pea. Physiologia Plantarum 79: 1-6, 1990

Sulfate ion effect on stability and regulatory properties of PEP carboxylase from the C4 plant Cynodon dactylon. Russian Journal of Plant Physiology 48(2): 176-180, 2001

Phylogenetic relationships in cynodon m part 2 cynodon nlemfuensis m cynodon dactylon var afghanicus m and cynodon dactylon var aridus m. Phyton (Buenos Aires) 28(2): 121-130, 1971

Some properties and physiological roles of phosphoenolpyruvate carboxylase in Rhodopseudomonas sp. No. 7 grown on ethanol under anaerobic-light conditions. Bioscience, Biotechnology, and Biochemistry 61: 5-30, 1997

Activation of maize (Zea mays L.) phosphoenolpyruvate carboxylase by glucose-6-phosphate and glycine: effects of pH and Mg2+. Photosynthetica 19(2): 177-182, 1985

Identification of proteins associated with water-deficit tolerance in C4 perennial grass species, Cynodon dactylon√óCynodon transvaalensis and Cynodon dactylon. Physiologia Plantarum 141(1): 40-55, 2011

Some properties and physiological roles of phosphoenolpyruvate carboxylase in Rhodopseudomonas sp. Number 7 grown on ethanol under anaerobic-light conditions. Bioscience Biotechnology & Biochemistry 61(4): 625-630, 1997