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Recognition of the nucleoside in the first position of the anticodon of isoleucine trna by isoleucyl trna synthetase from escherichia coli



Recognition of the nucleoside in the first position of the anticodon of isoleucine trna by isoleucyl trna synthetase from escherichia coli



Nucleosides & Nucleotides 11(2-4): 719-730



Escherichia coli tRNA1Ile prepared in vitro by T7 RNA polymerase transcription was found to be charged with isoleucine by E. coli isoleucyl-tRNA synthetase (IleRS). Replacement of G in the first position of the anticodon with U, C or A resulted in complete loss of isoleucine-accepting activity. This indicates that IleRS strictly recognizes the structural feature of guanosine in this position of tRNA1Ile which is common to that of lysidine in E. coli tRNA2Ile.

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