Restriction mapping of fragments of bacillus thuringiensis ssp israelensis plasmid dna cloned in escherichia coli
Takova Ts; Miteva, V.
Acta Microbiologica Bulgarica 23: 18-21
1988
ISSN/ISBN: 0204-8809 Accession: 007753509
Two recombinant plasmids-a result of cloning the Hind III fragments of the 72 MDa plasmid of Bacillus thuringiensis subsp. israelensis (BTI) in E. coli using the vector pUC 13 were mapped with restriction endonucleases. By mono, double and triple restrictions a map including five restriction sites was constructed.
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