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Ribosome assembly in salmonella typhimurium 7136 during recovery from heat shock



Ribosome assembly in salmonella typhimurium 7136 during recovery from heat shock



Food Microbiology 7(1): 53-68



Sublethal heat shock of Salmonella typhimurium cells (60 min at 48.degree.C, in 100 mM potassium phosphate buffer, pH 7.2 containing 50 mM EDTA) caused ribosome damage resulting in the formation of a 70S aggregate. This aggregate, lacking 16S ribosomal RNA, was formed by the non-specific binding of 30 S ribosomal proteins to the 50S subunit. Several ribosomal proteins were present in reduced amounts on heat damaged ribosomes and in heat shocked cells. Protein synthesis, inhibited by puromycin during cell recovery from heat shock, was required for ribosomal subunit and ribosomal RNA maturation. Ribosomal proteins, preferentially synthesized during cell recovery, were used to reconstruct the damaged ribosome. Ribosomal proteins which had dissociated from the damaged ribosome following heat shock did not fully participate in ribosome repair.

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