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S layer of lactobacillus helveticus atcc 12046 isolation chemical characterization and re formation after extraction with lithium chloride

S layer of lactobacillus helveticus atcc 12046 isolation chemical characterization and re formation after extraction with lithium chloride

Journal of General Microbiology 138(3): 611-618

In a previous study, electron microscopic examinations of thin sections of Lactobacillus helveticus ATCC 12046 revealed a three-layered structure of the cell wall. The outermost component was identified as a layer of a nonglycosylated 52 kDa protein. Freeze-etched preparations of intact cells have now demonstrated that this protein layer is an oblique surface layer (S-layer) lattice (a = 4.5 nm, b = 9.6 nm, .gamma. = 77.degree.) which completely covers the cell surface. Treatment with 5 M-LiCl extracted the S-layer protein from intact cells efficiently and selectively. Viability did not decrease significantly. Moreover, the S-layer reappeared when treated cells were allowed to grow again. In vitro self-assembly products obtained upon aggregation of isolated S-layer subunits exhibited the same oblique S-layer symmetry as observed on intact cells in vivo. The purified S-layer protein had a high content (44%) of hydrophobic amino acids. The N-terminal sequence was mainly composed of alanine, threonine, asparagine and aspartic acid.

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Accession: 007767177

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DOI: 10.1099/00221287-138-3-611

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