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Separation of myosin heavy chain isoforms in rat skeletal muscles by gradient sds polyacrylamide gel electrophoresis



Separation of myosin heavy chain isoforms in rat skeletal muscles by gradient sds polyacrylamide gel electrophoresis



Biomedical Research 11(2): 87-92



This study was conducted to investigate the electrophoretic condition for the separation of a novel myosin heavy chain isoform, designated as HCIId, in rat skeletal muscles by gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis. HCIId was separated on a polyacrylamide (5-8%)/glycerol (30-40%) gradient gel when electrophoresis was performed at 150 V for about 18 h at 8.degree. C. HCIId was observed in most fast-twitch muscles. Of those muscles, the deep portion of the masseter muscle contained the most abundant HCIId and one of the masseter muscles contained the neonatal-type MHC.

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