+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Simple free amino acid separation by reversed phase ion pair liquid chromatography using column switching technique



Simple free amino acid separation by reversed phase ion pair liquid chromatography using column switching technique



Journal of Liquid Chromatography 11(8): 1741-1751



Reversed-phase ion-pair liquid chromatography with copper (II) ions (RP-IPC-Cu) was applied to develop a high speed separation of free amino acids. Dynamic gradient elution in RP-IPC-Cu could not achieve this purpose due to base line shift, therefore a column switching technique was used with a combination of different alkyl-bonded silica gel columns under isocratic elution. The flexibility of eluent components makes it easy to realize a rapid separation of a mixture of targeted amino acids.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007793444

Download citation: RISBibTeXText

DOI: 10.1080/01483918808076734


Related references

Separation of free amino acids by reversed phase ion pair chromatography with column switching and isocratic elution. Journal of Chromatography 507: 95-102, 1990

Determination of S-carboxymethylcysteine in serum by reversed-phase ion-pair liquid chromatography with column switching following pre-column derivatization with o-phthalaldehyde. Journal of Chromatography 428(2): 301-310, 1988

Separation of purine bases, nucleosides and nucleotides by a column-switching technique combining reversed-phase and anion-exchange high-performance liquid chromatography. Journal of Chromatography 356(1): 115-122, 1986

Orthogonal separation on one beta-cyclodextrin column by switching reversed-phase liquid chromatography and hydrophilic interaction chromatography. Talanta 81(4-5): 1870-1876, 2010

Monolithic column-based reversed-phase liquid chromatography separation for amino acid assay in microdialysates and cerebral spinal fluid. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 848(2): 323-328, 2007

Monolithic alkylsilane column: A promising separation medium for oligonucleotides by ion-pair reversed-phase liquid chromatography. Journal of Chromatography. a 1569: 168-177, 2018

Complete two-dimensional separation for analysis of acidic compounds in plasma using column-switching reversed-phase liquid chromatography. Journal of Chromatography B 726(1-2): 225-236, 1999

Comparison of ion-pair and amide-based column reversed-phase liquid chromatography for the separation of thiamine-related compounds. Journal of Chromatography B 757(2): 301-308, 15 June, 2001

Separation of 5-fluorouracil and uracil by ion-pair reversed-phase high-performance liquid chromatography on a column with porous polymeric packing. Journal of Chromatography 307(2): 464-468, 1984

Determination of chlorophylls by reversed-phase high-performance liquid chromatography with isocratic elution and the column-switching technique. Journal of Chromatography A 693(1): 176-180, 1995

Simple rapid and highly efficient separation of amino acid phenyl thio hydantoins by reversed phase high performance liquid chromatography. Analytical Biochemistry 121(2): 281-285, 1982

Separation of collagen hydrolysate amino acids by ion pair reversed phase high performance liquid chromatography. Journal of Chromatography 364: 253-258, 1986

Separation of several free polyamines and their acetylated derivatives by ion-pair reversed-phase high performance liquid chromatography. Biomedical Chromatography 12(5): 291-293, 1998

Automated determination of amoxicillin in biological fluids by column switching in ion pair reversed phase liquid chromatographic systems with post column derivatization. Journal of Chromatography Biomedical Applications 344: 285-296, 1985

Separation of p-aminobenzenearsonic acid and its oxide by ion-pair reversed-phase high performance liquid chromatography. Se Pu 17(3): 275-277, 1999