Small cell lung cancer and neuroendocrine cell surface antigen defined by two monoclonal antibodies

Kunii, T.

Journal of Tokyo Medical College 47(2): 274-283


Accession: 007798932

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Two monoclonal antibodies (NCC-LU-246 : IgG1, NCC-LU-243 : IgG2a) were obtainied by using a human small cell lung cancer xenograft, Lu-24 as an immunogen. Immunohistochemically, two monoclonal antibodies were reactive with small cell lung cancer, but not with non-small cell lung cancer or other carcinomas apart from neuroblastoma and bronchial carcinoid. Among the normal cells, brain, peripheral nerve, thyroid gland, Langerhans islets, cardiac muscle, adrenal gland reacted with the antibodies, but epithelium and bone marrow cells did not. Thus, the expression of NCC-LU-246 and NCC-LU-243 antigens is associated with neuroendocrine differentiation. The molecular weight of NCC-LU-246 and NCC-LU-243 antigens estimated by radioimmunoprecipitation was 145 KDa. Inhibition assay and anti-idiotype polyclonal antibodies analysis showed that these two monclonal antibodies defined different epitope of the same antigen. These two monoclonal antibodies could be useful for cytological diagonsis of small cell lung cancer and biological research.