+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Staining of bone canaliculi using decalcified bone tissues

Staining of bone canaliculi using decalcified bone tissues

Nihon Seikeigeka Gakkai Zasshi 65(9): 757-761

Recently we were able to stain bone canaliculi using the decalcified bone tissues, according to a modified method of the Bodian staining method, which is commonly used for staining the nerve fibers with silver impregnation of protargol. The bone tissues from normal rabbits, rats, and humans were decalcified with buffered EDTA solution after 10% formalin fixation. The tissues were embedded in paraffin and were sliced at 4 microns then the sections were attached to the gelatin-coated glass slides. These sections were treated first with potassium dichromate followed by 2% protargol solution containing copper. After gold chrolide treatment, they were immersed in oxalic acid. Both the lacunae and canaliculi of osteocytes were stained black in sharp contrast to bone matrix of red purple. Using the present staining method, the bone canaliculi were clearly detectable in fine details without producing apparent artificial damage of soft tissues. The canaliculi showed also markedly complex fine structures. By combining the present staining method with eosin or toluidine blue staining, we were able to recognize even immature osteocytes in enchondral ossification at growth cartilage with fine bone canaliculi. From these results, we conclude that the present method is very useful in histopathological studies of the lacuna-canalicular system of osteocytes in the decalcified bone tissues.

(PDF emailed within 1 workday: $29.90)

Accession: 007816456

Download citation: RISBibTeXText

PMID: 1720446

Related references

The development of bone canaliculi in the endochondral or intramembranous ossification analyzed by histological study of decalcified bone using silver staining method. Journal of Kyoto Prefectural University of Medicine 100(8): 801-818, 1991

Differential Staining Of Bone Tissue Elements By Silver Nitrate Staining Of Blocks Of Decalcified Bone. Anatomical Record 151: 125-131, 1965

The demonstration of canaliculi in sections of decalcified bone by a silver impregnation method. Stain Technology 52(5): 301-302, 1977

A staining method for bone canaliculi. Acta Orthopaedica Scandinavica. 66(2): 166-168, 1995

On the staining technique of fresh-non-decalcified bone sections. Zeitschr Mikrosk Anat Forsch 64(4): 634-636, 1958

Variation of the Holmes method of histologic staining of bone canaliculi. Journal of Histotechnology 16(4): 355-357, 1993

Use of Masson's trichrome method for staining decalcified bone tissue. Arkhiv Patologii 58(1): 66-67, 1996

Preparation of decalcified bone matrix, bone cement and bone morphogenetic protein composite. Zhonghua Yi Xue Za Zhi 81(22): 1374-1376, 2002

Block staining with hematoxylin, gelatin embedding and serial sectioning of decalcified bone. Stain Technology 37: 161-163, 1962

The staining of decalcified histological bone slices with the improved Romanowsky-Giemsa solution. Zeitschrift für Mikroskopisch-Anatomische Forschung 104(2): 202-211, 1990

A technique for the simultaneous staining of osteocytes and osteons in frozen sections of decalcified bone. Stain Technology 55(1): 47-48, 1980

Augmentation of bone healing in delayed and atrophic nonunion of fractures of long bones by partially decalcified bone allograft (decal bone). Indian Journal of Orthopaedics 49(6): 637-642, 2016

Correspondence of silver nitrate staining patterns in decalcified bone withe the microradiographic image. Anatomical Record 182(2): 267-273, 1975

A contribution to the staining of decalcified histological slices of bone with the reformed romanowsky giemsa solution. Zeitschrift fuer Mikroskopisch-Anatomische Forschung (Leipzig) 104(2): 202-211, 1990