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Stimulating effect of elevated temperature treatments on production of meristemoids from pollen callus of tea camellia sinensis l. o. kuntze



Stimulating effect of elevated temperature treatments on production of meristemoids from pollen callus of tea camellia sinensis l. o. kuntze



Indian Journal of Experimental Biology 30(2): 83-86



Effect of post-inoculation exposure of anthers to cold and elevated temperatures was studied to achieve regeneration of androgenic plants in tea. Cold temperature treatments failed to show any positive response, however, elevated temperature treatment at 35.degree. C for a period of 5 days significantly stimulated the yield of shoot apices originating from the intervening pollen calli of tea anthers cultured on agar gelled B5 medium containing (mg/l): 2,4 - D, 0.1; NAA, 0.1; kinetin, 0.1; glutamine, 400 and sucrose 7% at pH 5.5.

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