Studies on alkaline phosphatase activity in amphibians iii. effects of inhibitor and temperature on alkaline phosphatases in japanese amphibians
Scientific Report of the Laboratory for Amphibian Biology Hiroshima University 10: 243-254
The effects of L-homoarginine, L-phenylalanine, L-phenylalanylglycylglycine and L-leucine and high temperatures on the alkaline phosphatases extracted from the skins, lungs, livers, kidneys, ovaries and small intestines of Rana nigromaculata, Rana japonica, Rana rugosa, Rana limnocharis, Rana catesbeiana, Rhacophorus schlegelii, Hyla arborea japoncia, Bufo japonicus and Cynops pyrrhogaster were examined in order to confirm the differences in the stability of alkaline phosphatases to these inhibiting-agents. The alkaline phozphatases of the skins, lungs, livers, kidneys and ovaries from all species, as well as those of the small intestines from Rana nigromaculata, Rana japonica, Rana, rugosa, Rana limnocharis, Rana catesbeiana and Rhacophorus schlegeli required nearly the same concentrations of inhibitors for 50% inhibition, while the alkaline phosphatases of the small intestines from Hyla arborea japonica, Bufo japonicus and Cynops pyrrhogaster required remarkably higher concentrations of inhibitors for 50% inhibition than the foregoing alkaline phosphatases. The alkaline phosphatases of all the organs from Rana limnocharis other than the small intestine were remarkably temperature-resistant.