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Chapter 7,832

Studies on biological tissue electrode sensitive to cytochrome C

Shen, G.L.; Li, Y.Z.; Zeng, G.M.; Yu, R.Q.; Xie, J.Y.; Zhang, M.F.

Yao Xue Xue Bao 23(10): 762-766

1988

ISSN/ISBN: 0513-4870
PMID: 2855773
Accession: 007831161
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A tissue section electrode sensitive to cytochrome (c) has been made by using a thin slice of porcine kidney coupled with an oxygen electrode. Over the concentration range of 0.01 to 0.227 mg/ml of cytochrome c, the dynamic response of electrode is linearly related to the logarithm of the concentration of cytochrome c and the correlation coefficient is -0.9949. Some experimental factors such as buffer composition, pH, temperature, tissue slice thickness and preservative agent were investigated in detail. The probe is usable for up to 1 .apprx. 2 weeks. the average recovery of cytochrome c assay determined by the dynamic response method was 98.8%, with a standard deviation of 3.4%.

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