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Studies on compartmentation of uridine 5' triphosphate during synthesis of cytoplasmic and plastid ribosomal rna's in euglena gracilis



Studies on compartmentation of uridine 5' triphosphate during synthesis of cytoplasmic and plastid ribosomal rna's in euglena gracilis



International Journal of Biochemistry 23(11): 1219-1224



1. Compartmentation of uridine-5'-triphosphate (UTP) was studied during synthesis of cytoplasmic ribosomal RNA (cyt-rRNA) and plasma ribosomal RNA (pl-rRNA) in photoorganotrophically grown cells of Euglena gracilis Z. 2. Using the approach of Weigers et al. (1976) the steady state specific radioactivity of UTP was compared with that of cyt-20S rRNA, cyt-25S rRNA, pl-16S rRNA and pl-23S rRNA under low and at 100-fold higher specific radioactivity of exogenously fed [3H]-uracil. 3. The equal steady state specific radioactivities of all rRNAs at both feeding conditions argue against compartmentation of UTP during their synthesis. 4. At high specific radioactivity of exogenous [3H]-uracil the salvage-derived labelled UMP was shown to be diluted 15,000-fold by unlabelled UMP formed de novo, whereas this dilution factor was 100-fold lower at low specific radioactivity of [3H]-uracil indicating inhibition of the de novo synthesis of UMP. 5. Transport is suggested of uridine nucleotides into chloroplasts by the 15-fold higher specific radioactivity of intracellular [3H]-uracil than that of UTP as well as UMP residues in pl-rRNA.

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