Studies on the biological function of the nucleotide base of vitamin B12
Eberhard, G.; Schlayer, H.; Joseph, H.; Fridrich, E.; Utz, B.; Müller, O.
Biological Chemistry Hoppe-Seyler 369(10): 1091-1098
ISSN/ISBN: 0177-3593 PMID: 2907405 Accession: 007833925
Studies on the coenzyme activity of some nucleotide base analogues of adenosylcobalamine in the dioldehydratase and methylmalonyl-CoA mutase systems resulted in fundamental differences: Contrary to dioldehydratase the coenzyme activity remains in the methylmalonyl-CoA mutase system when adenosylcobamides with a strongly modified nucleotide base are studied. The analogues give a good growth response with Escherichia coli. In the growth test with Lactobacillus leichmanii only the cobamides that do not have substituted nucleotide bases in the positions 2 or 3 of their imidazole ring are active.