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Studies on the chromatographic behavior of some uronic acids and neutral sugars on an amino bonded phase column



Studies on the chromatographic behavior of some uronic acids and neutral sugars on an amino bonded phase column



Journal of Chromatography 513: 227-236



The differences in the chromatographic behaviour of glucuronic and galacturonic acids and some common neutral sugars on an amino-bonded phase column (LiChrosorb NH2) were studied. The mobile phase was either acetonitrile-water or acetonitrile-sodium dihydrogenphosphate buffer. The influences of the presence of an anion (H2PO4-) in the mobile phase, the pH and the composition of eluent were investigated. The results contributed to the elucidation of the mechanism of the elution of uronic acids and the simultaneous determination of uronic acids and neutral sugars.

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Accession: 007834187

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DOI: 10.1016/s0021-9673(01)89439-5


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