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Successive inoculation of lucerne plants with causal agents of bacterial and verticillium wilt



Successive inoculation of lucerne plants with causal agents of bacterial and verticillium wilt



Sbornik UVTIZ (Ustav Vedeckotechnickych Informaci pro Zemedelstvi) Ochrana Rostlin 26(2): 123-126



Lucerne (Medicago sativa L.) plants of seven new varieties and Palava variety were inoculated in glasshouse conditions: 1. at the stage of cotyledonous leaves with Clavibacter michiganensis subp. insidiosus (Cmi) bacteria; 2. at the age of nine weeks with the Verticillium albo-atrum (Vaa) fungus; 3. at the stage of cotyledonous leaves with Cmi bacteria and at the age of nine weeks the same plants were inoculated with the Vaa fungus. In comparison with the inoculation with one pathogen, after successive inoculation with the two pathogens, the proportion of susceptible plants was on an average 11% higher (within the range of 3-11%). It is not enough to determine separately the resistance to Cmi and Vaa in the areas with the occurrence of both bacterial and verticillium wilt. It is also necessary to know the proportion of plants which have the complex resistance to both pathogens.

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