Sucrose metabolizing enzymes from leaves of barley infected with brown rust puccinia hordei otth

Tetlow, I.J.; Farrar, J.F.

New Phytologist 120(4): 475-480

1992


ISSN/ISBN: 0028-646X
DOI: 10.1111/j.1469-8137.1992.tb01795.x
Accession: 007844249

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Abstract
The capacity for sucrose synthesis in primary leaves of barley (Hordeum vulgare L.) infected with brown rust (Puccinia hordei Otth.) was assessed by measuring the activity of sucrose phosphate synthase (SPS). SPS activity gradually declined during rust infection to 32% of uninfected controls by the sporulation stage. Extractable SPS activity was constant in the light period in both uninfected and infected (sporulating) leaves; activity then declined in the first hour of darkness by 30% in uninfected and 60% in sporulating leaves until returning to the original activity at the start of the following light period. The activity of acid invertase (AI) increased more than four-fold in rust-infected (sporulating) primary leaves compared with controls. Extracellular AI activity in rusted tissue was two-fold greater than in healthy. The consequences of the changes in enzyme activities in the diseased plant are discussed in relation to export from the diseased leaf.

Sucrose metabolizing enzymes from leaves of barley infected with brown rust puccinia hordei otth