+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Suitability of bactoscan for the estimation of the bacteriological quality of raw milk

Suitability of bactoscan for the estimation of the bacteriological quality of raw milk

Milchwissenschaft 43(9): 577-580, 585-586

In the second half of 1987 the bacteriological quality of about 2000 samples of raw milk was estimated with the Bactoscan. The standard deviation of repeatability (sr) was about 0.031 log Bactoscan units, in agreement with the specifications of the instrument and much lower than the sr of the traditional plate count method (0.069 log plate count units). No influence of the plate count level on the sr of the Bactoscan count could be detected. Further, the Bactoscan count was compared with the plate count. From the statistical point of view it was very difficult to analyze the data in a theoretically sound way. With this restriction in mind it can be stated that above a plate count of about 30,000 cfu /ml a linear relationship between log plate count and log Bactoscan count could be established with an (sy)x (standard deviation of the estimate of the log plate count from the log Bactoscan count) of about 0.23 log plate count units. Using this regression line to classify the samples according to the Dutch payment system, a classification could be made which was almost equal to the classification by the plate count. First class: 91.57% (Bactoscan) vs. 90.65% (plate count); second class: 6.28% vs. 6.38%; third class: 2.15% vs 2.96%. When milk samples with a plate count < 30,000 cfu/ml were included in the calculations, the regression line becomes curved due to the background noise of the milk itself. Multiple polynomial regression analysis revealed that the influence of the number of somatic cells on the Bactoscan count is negligible. The carry over between sample and blank was about 1%. An experiment with milk samples treated with a syringe to disperse clumps or chains of bacterial cells showed an increased plate count when compared with the untreated samples, whereas the Bactoscan count did not increase. Long term stability of the instrument appeared to be excellent during the half year of testing and could easily be controlled by using the standards of Foss Electric (Hillerod, Denmark), when given due care. From the experiments, no evidence was found for systematic deviations from the regression line, due to individual milk suppliers.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 007844859

Download citation: RISBibTeXText

Related references

Measuring the bacteriological quality of raw milk with the Bactoscan apparatus. First experiences and results with the Bactoscan III system. Deutsche Molkerei Zeitung 109(44;46): 1436, 1438-1440, 1442-1446;1551-1555, 1988

Determining the bacteriological quality of raw milk with the Bactoscan (Version 2/87). Deutsche Molkerei Zeitung 109(13): 380-381, 384, 386-387, 1988

Estimating bacteriological quality of raw milk - predictive value of the Bactoscan method. DMZ, Lebensmittelindustrie und Milchwirtschaft 112(8): 188-192, 1991

The Bactoscan procedure for determination of bacteriological quality of delivery milk. Deutsche Milchwirtschaft 36(39): 1282-1284, 1985

Bacteriological quality of raw milk: Conversion of Bactoscan-FC counts onto the scale of the official method. Milchwissenschaft 56(7): 380-384, 2001

The Baden-Wurttemberg and Hesse Lands have determined the bacteriological quality of delivery milk by the Bactoscan procedure. Deutsche Molkerei Zeitung 105(14): 424, 426-429, 1984

Bacteriological raw-milk quality: factors influencing the relationship between colony-forming units and Bactoscan-FC counts. Milchwissenschaft 60(1): 28-31, 2005

Paying for milk according to its bacteriological quality. Instructions for estimation of the bacteriological quality of raw milk for payment by dairies (non-refrigerated milk). Revue lait. fr, 283, 23-27, 1971

Determination of the bacteriological quality of raw milk by automatic fluorescent microscopic counting of single bacteria (Bactoscan 8000) - a review. Kieler Milchwirtschaftliche Forschungsberichte 49(3): 163-186, 1997

The suitability of the bactoscan method for grading raw milk samples. Meijeritieteellinen Aikakauskirja 42(1): 33-43, 1984

Suitability of the Bactoscan apparatus for determination of total cell count in raw milk. Milchwirtschaftliche Berichte aus den Bundesanstalten Wolfpassing und Rotholz (76): 211-215, 1983

Suitability of the Bactoscan procedure for determination of total bacterial count of raw milk. Osterreichische Milchwirtschaft 38(19): 373-384, 1983

Suitability of Bactoscan device for determining the total germ count in raw milk. Milchwirtschaftliche Berichte aus den Bundesanstalten Wolfpassing und Rotholz: (76) 211-215, 1983

Evaluation of bacteriological status of raw milk using the direct count (Bactoscan) procedure. Molkerei Zeitung Welt der Milch 36(42): 1325-1328, 1982

Suitability of the nitrate test in estimating the bacteriological quality of milk.. Karjantuote. 17, 59-65, 1962