+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Tachykinins mediate bronchoconstriction elicited by isocapnic hyperpnea in guinea pigs



Tachykinins mediate bronchoconstriction elicited by isocapnic hyperpnea in guinea pigs



Journal of Applied Physiology 66(3): 1108-1112



We tested the hypothesis that tachykinins mediate hyperpnea-induced bronchoconstriction (HIB) in 28 guinea pigs. Stimulus-response curves to increasing minute ventilation with dry gas were generated in animals depleted of tachykinins by capsaicin pretreatment and in animals pretreated with phosphoramidon, a neutral metalloendopeptidase inhibitor. Sixteen anesthetized guinea pigs received capsaicin (50 mg/kg sc) after aminophylline (10 mg/kg ip) and terbutaline (0.1 mg/kg sc). An additional 12 animals received saline (1 ml sc) instead of capsaicin. One week later, all animals were anesthetized, given propranolol (1 mg/kg iv), and mechanically ventilated (6 ml/kg, 60 breaths/min, 50% O2 in air fully water saturated). Phosphoramidon (0.5 mg iv) was administered to five of the noncapsaicin-treated guinea pigs. Eucapnic dry gas (95% O2-5% CO2) hyperpnea "challenges" were performed by increasing the tidal volume (2-6 ml) and frequency (150 breaths/min) for 5 min. Capsaicin-pretreated animals showed marked attenuation in HIB, with a rightward shift of the stimulus-response curve compared with controls; the estimated tidal volume required to elicit a twofold increase in respiratory system resistance (ES200) was 5.0 ml for capsaicin-pretreated animals vs. 3.7 ml for controls (P less than 0.03). Phosphoramidon-treated animals were more reactive to dry gas hyperpnea compared with control (ES200 = 2.6 ml; P less than 0.0001). Methacholine dose-response curves (10(-11) to 10(-7) mol iv) obtained at the conclusion of the experiments were similar among capsaicin, phosphoramidon, and control groups. These findings implicate tachykinin release as an important mechanism of HIB in guinea pigs.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007862949

Download citation: RISBibTeXText

PMID: 2496085

DOI: 10.1152/jappl.1989.66.3.1108


Related references

Bronchoconstriction elicited by isocapnic hyperpnea in guinea pigs. Journal of Applied Physiology 65(2): 934-939, 1988

Oxygen radicals in bronchoconstriction of guinea pigs elicited by isocapnic hyperpnea. Journal of Applied Physiology 74(2): 627-633, 1993

Effects of endothelin B receptor antagonists, BQ788 and ET-1(11-21) fragment on the bronchoconstriction elicited by isocapnic hyperpnea in guinea pigs. Chinese Medical Journal 113(3): 217-221, 2002

Tachykinins participate in dry air hyperpnea induced bronchoconstriction hib in guinea pigs. American Review of Respiratory Disease 137(4 PART 2): 201, 1988

Tachykinins mediate hypocapnia-induced bronchoconstriction in guinea pigs. Journal of Applied Physiology 67(6): 2454-2460, 1989

Tachykinins mediate the potentiation of antigen-induced bronchoconstriction by cold air in guinea pigs. Journal of Allergy and Clinical Immunology 97(3): 756-760, 1996

Role of tachykinins in hyperpnea-induced bronchovascular hyperpermeability in guinea pigs. Journal of Applied Physiology 70(1): 27-35, 1991

Tachykinins mediate hypocapnia induced bronchoconstriction hibc in guinea pig lungs. American Review of Respiratory Disease 139(4 PART 2): A235, 1989

Tachykinins mediate hypocapnia induced bronchoconstriction hibc in postmortem guinea pig lungs. Australian & New Zealand Journal of Medicine 18(3 SUPPL 2): 537, 1988

Mediators in hyperpnea-induced bronchoconstriction of guinea pigs. Naunyn-Schmiedeberg's Archives of Pharmacology 360(5): 597-602, 1999

Time course of bronchoconstriction induced by dry gas hyperpnea in guinea pigs. Journal of Applied Physiology 70(2): 504-510, 1991

Ontogeny of dry gas hyperpnea-induced bronchoconstriction in guinea pigs. Journal of Applied Physiology 76(3): 1150-1155, 1994

Hyperpnea induced bronchoconstriction hib in immature guinea pigs. Pediatric Research 27(4 PART 2): 360A, 1990

Thermal recovery elicits bronchoconstriction after dry gas hyperpnea in guinea pigs. American Review of Respiratory Disease 139(4 PART 2): A233, 1989

Distribution of airway narrowing during hyperpnea-induced bronchoconstriction in guinea pigs. Journal of Applied Physiology 69(4): 1323-1329, 1990