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The adsorption of different types of monoclonal immunoglobulin on positively and negatively charged polystyrene latices

The adsorption of different types of monoclonal immunoglobulin on positively and negatively charged polystyrene latices

Colloids and Surfaces 54(1-2): 89-102

Electrostatic aspects of the interaction between monoclonal mouse anti-human chorionic gonadotropin immuno gamma globulins and polystyrene latex particles are reported. Adsorption and electrophoresis experiments have been performed as a function of pH, using both negatively and positively charged latices and monoclonal immuno gamma globulins having different isoelectric points. Adsorption isotherms show high affinity character with well-defined plateaus. The plateaus vary with pH, showing a maximum at the isoelectric point of the protein-covered polystyrene particles, rather than at the isoelectric point of the immuno gamma globulin in solution. Since a similar behaviour has been previously reported for bovine serum albumin this observation might be a more general feature in protein adsorption.

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Accession: 007871327

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DOI: 10.1016/0166-6622(91)80052-p

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