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The effect of nitrate fertilizer treatments upon the ultrastructure of lolium perenne l. chloroplasts

The effect of nitrate fertilizer treatments upon the ultrastructure of lolium perenne l. chloroplasts

Revue Roumaine de Biologie Serie de Biologie Vegetale 33(2): 111-114

The effect of nitrate fertilizers upon the ultrastructure of Lolium perenne L. chloroplast was studied in different doses: a) suboptimum (100 kg. ha-1); b) optimum (200 and 400 kg. ha-1) and c) superoptimum (800 kg. ha-1). It has been ascertained that the suboptimum dose determines few changes in the ultrastructure; the optimum doses do determine some starch grains accumulation in the chloroplast, as well as the increase of the granae/chloroplast number; the super-optimum dose proved to be toxic, because of the ammonium ion in the composition of the administered fertilizer, fact that became obvious by the chloroplast ultrastructure deterioration. On the basis of the microphotographs by electron microscopy, the hypothesis has been advanced that the ammonium nitrate (the experimented fertilizer), by the effect of the ammonium ion, determines an alteration of sugars' metabolism in the chloroplast.

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