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The effect of sputum contamination on the growth of mycobacterium kansasii mycobacterium avium and mycobacterium intracellulare

The effect of sputum contamination on the growth of mycobacterium kansasii mycobacterium avium and mycobacterium intracellulare

Igiene Moderna 97(1): 131-140

The authors tested the sensibility of M. avium intracellulare and M. tuberculosus to the habitual sputa decontamination process (Kubica's methos and Tacquet's method) and others. For it they used 34 strains from the Cordoba Medical School Mycobacterium's Collection, using the "artificial sputum" proposed by The German Society of Hygiene and Microbiology. They have compared the strains inhibited by the decontamination methods used. The three species was inhibited by Kubica's method (12.50 .+-. 11.69 and 15.38 .+-. 10.01 respectively). However, there were not significant differences between the seven methods tested. One of the most used sputa decontamination processes, Kubica's method, is very aggressive in the experimental decontamination of "artificial sputa". According to our results 14.65% of strains are not recovered after the decontamination.

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