The effect of trace amounts of tissue factor on thrombin generation in platelet rich plasma, its inhibition by heparin

Béguin, S.; Lindhout, T.; Hemker, H.C.

Thrombosis and Haemostasis 61(1): 25-29


ISSN/ISBN: 0340-6245
PMID: 2749591
Accession: 007893318

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Amounts of human brain thromboplastin that do not stimulate thrombin generation in platelet poor plasma, were shown to advance by about 4 min an explosive formation of thrombin that occurs after recalcification in the presence of blood platelets. This synergistic effect is inhibited by the specific thrombin inhibitor hirudin and mimicked by adding low concentrations (less than 5 nM) of thrombin to platelet rich plasma. It is our conclusion that small amounts of thrombin, generated under the influence of thromboplastin induced procoagulant activity in the blood platelets. This activity is most likely mainly due to procoagulant phospholipids. Heparin inhibits this effect and retards the explosive thrombin formation. It does not, however, diminish the peak amount of thrombin eventually formed, because heparin neutralizing material released from the activated platelets quenches the heparin effect.