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The establishment of persistent infection of herpes simplex virus in raji cell and the effects of tnf ifns and antiserum to it


The establishment of persistent infection of herpes simplex virus in raji cell and the effects of tnf ifns and antiserum to it



Virologica Sinica 5(3): 278-284



ISSN/ISBN: 1674-0769

Persistent infection of herpes simplex virus (HSV) had been maintained in human B lymphoblastoid (Raji) cells for 150 days. The whole course of the persistent infection can be divided into two phases: acute (within 30 days p.i.) and persistent stable (30 days later p.i.). In the acute phase, the virus in the supernatant reached 10602TCID50/ml, HSV antigen positive cells (HSV Ag+ cell) detected by immunofluorescence reached 21% and the percentage of dead cells reached 42%. In the stable phase, the virus and Raji cells became relatively balanced: virus in the supernatant ranged 103.5-4.2 TCID50/ml, HSV Ag+ cell ranged 5-10% and the dead cell was less 5% (the same as controls). Treating the cell culture with recombinant interferons (rIFN.alpha.,rINF.gamma.), recombinant interleukin 2(rIL-2) and tumor necrosis factor (TNF) respectively, we found that TNF and rIFN.alpha. inhibited the virus replication markedly while so did rIFN.gamma. but weakly and rIL-2 had no effects. Removing the factors 5 days later, the virus and HSV Ag+ cells gradually recovered as controls. Treating the cultures with anti-HSV serum, the virus and Ag+ cells were diminished and undetectable. After removing the antibody, the virus and its Ag+ cells no longer repeated and so did continously for 50 days. These studies provided a useful model for research on HSV persistent infection in vitro in many aspects.

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