The production of folic acid by microorganisms isolated from fermenting corn meal

Yoa, F.G.; Fields, M.L.; Chung, H.J.

Korean Journal of Applied Microbiology and Bioengineering 16(5): 352-357

1988


Accession: 007925663

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Abstract
Twenty-five out of 35 strains isolated from fermented corn meal produced folic acid. Bacillus licheniformis strain 6 and Enterobacter cloacae strain 18 produced the largest quantities of 1830 .+-. 271 ng and 1350 .+-. 167 ng per 100 ml of the assay broth, respectively. B. licheniformis produced maximum yields when initial pH valves were 6, 7, or 8 and were incubated at 35.degree. C for 5 days. The initial pH (range 4-8) had no effect on folic acid production by E. cloacae; 35.degree. C for 5 days was optimal for this bacterium. Added carbohydrates had no effect on the production of total folic acid in the bacterial cells in pure or mixed cultures. However, in the growth media, carbohydrates enhanced the production of free and total folic acid by E. cloacae and in the mixed cultures. Added carbohydrates had no significant (P < 0.05) effect on the production of free and total folic acid by B. licheniformis.