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The relative affinity of recombinant dihydrolipoamide transacetylase for autoantibodies in primary biliary cirrhosis

, : The relative affinity of recombinant dihydrolipoamide transacetylase for autoantibodies in primary biliary cirrhosis. Hepatology 11(5): 717-722

In normal individuals there is an adaptive immune response to a foreign antigen in which antibodies of increasing affinity are produced with time. This is not always true of an autoimmune response. However, because only a limited number of autoantigens have been cloned or purified, this issue has not been studied well. In primary biliary cirrhosis the predominant manifestation of autoimmunity is antimitochondrial antibodies that react with dihydrolipoamide transacetylase. The availability of recombinant dihydrolipoamide transacetylase and the development of a rapid and reproducible enzyme-linked immunosorbent assay for autoantibodies has allowed us to address the affinity of autoantibodies using thiocyanate inhibition. Thiocyanate is a chaotropic compound known to inhibit antigen-antibody binding in a concentration-dependent manner. We used this property to inhibit the binding by enzyme-linked immunosorbent assay of human recombinant dihydrolipoamide transacetylase with serum autoantibodies from 55 patients with primary biliary cirrhosis. The relative affinity and serum autoantibody titers were then compared with the histological stage of the liver biopsy sample. Interestingly, we found a considerable heterogeneity of relative affinities. These relative affinities did not correlate with the histological stage or the serum titer of antimitochondrial antibodies. However, the ability of serum autoantibodies to inhibit intact primary biliary cirrhosis enzyme activity was found to correlate highly (R2 = 0.751) with the relative affinity. Thus there are profound differences between patients with respect to qualitative expression of autoantibodies. The significance of this data will be unclear until more is determined regarding the nature of the epitope that drives T cells and leads to B-cell responses.

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Accession: 007929353

PMID: 2347544

DOI: 10.1002/hep.1840110502

PDF Full Text: The relative affinity of recombinant dihydrolipoamide transacetylase for autoantibodies in primary biliary cirrhosis

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Vigneresse Jean L., 1987: Three-dimensional organization of the Echassieres Massif and synthesis of surface geophysical measurements. Geologie de la France 1987(2-3): 27-31

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Dikken, J.L.; van de Velde, C.J.H.; Coit, D.G.; Shah, M.A.; Verheij, M.; Cats, A., 2012: Treatment of resectable gastric cancer. Stomach cancer is one of the most common cancers worldwide, despite its declining overall incidence. Although there are differences in incidence, etiology and pathological factors, most studies do not separately analyze cardia and noncardia gastri...

Conway, John R., 1996: Nuptial, pre-, and postnuptial activity of the thatching ant, Formica obscuripes Forel, in Colorado. Observations and excavations of thatching ant nests from 1990 to 1994 at 2560 m in Colorado provided information on the numbers and behavior of males and winged and wingless queens. Nuptial activity was compared to that reported by other investiga...

Ailor, E.; Betenbaugh, M.J., 1996: Application of cytosolic chaperones to improve recombinant protein production in insect cells. Abstracts of Papers American Chemical Society 211(1-2): BIOT 95

Crabill, R. E. Jr, 1976: A new watophilus from utah usa including a list of all known species chilopoda geophilomorpha chilenophilidae. Watophilus includes 6 spp.: W. alabamae Chamberlin (known from Georgia and South Carolina); W. errans Chamberlin and W. laetus Chamberlin (both species known from Berkeley, California, only); W. utus Chamberlin (La Sal Mountains, San Juan Co., Uta...

Reed, R.J.; Webb, S.V.; Clark, W.H., 1990: Minimal deviation melanoma (halo nevus variant). Twenty-eight cases of minimal deviation melanoma (MDM) in the histologic setting of halo nevus formed the basis for this study. Requisites for this study included at least one of the following: a remnant of a halo nevus in a MDM; remnants of both...

Stearn Colin William, 1961: Devonian stromatoporoids from the Canadian Rocky Mountains. In the carbonate facies of the Fairholme Group (Upper and possibly Middle Devonian) stromatoporoids are important rock-forming and reef-building organisms. A fauna from these beds and from the overlying Alexo and Palliser formations is described....

Schulman M.P.; Andersson B.; Orrenius S., 1977: Factors altering the rate of alcohol metabolism in hepatocytes. Thurman, Ronald G Et Al (Ed ) Alcohol And Aldehyde Metabolizing Systems, Vol Iii Intermediary Metabolism And Neurochemistry Second International Symposium Philadelphia, Pa , Usa , Oct 15-17, 1976 Xxv+673p Illus Academic Press, Inc : New York, N Y , Usa; London, England Isbn 0-12-691403-6 355-336

Vandova, M.G.igorov, I.D.chev, D., 1983: Dependence of peach development rate on temperature conditions. II. Sliven ecological region. Gradinarska i lozarska nauka = Horticultural and viticultural science0(7): 31-39