+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The sporadic change of epiblast cells to epithelial in the chick embryo before the formation of hensen's knot



The sporadic change of epiblast cells to epithelial in the chick embryo before the formation of hensen's knot



Zoological Science 8(5): 869-878



33 chick embryos from stage 2 to stage 4 according to Hamburger and Hamilton [1] were stained in toto with Rhodamine phalloidin (Rh-P) and then surveyed with a fluorescence microscope. Two types of cells were stained: one, elongated and stained along a whole cell surface, another, not elongated, stained eminently along the polygonal cell border at the upper surface. The former, recognized at or along the growing primitive streak (PS), were considered as the mesodermal cells because of their flask-like shape and downward inclination. The latter, appearing at the later stage of PS formation, were considered as the epithelial cells because of the polygonal frame work of F-actin along the cell border. At first, a few epithelial cells clustered to form small islands scattering along and slightly apart from the PS. The islands then increased in both number and size, becoming connected to each other forming two belts along the PS. When Hensen's knot was formed, all epiblastic cells differentiated into epithelial cells with the polygonal framework of F-actin.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 007936798

Download citation: RISBibTeXText


Related references

Individual epiblast cells acquired invasiveness precedent to the primitive streak formation in the chick embryo. Zoological Science 14(2): 313-320, 1997

When does the epiblast establish epithelial structure in the chick embryo. Zoological Science 5(6): 1257, 1988

Somite formation in the early chick embryo following grafts of Hensen's node. Journal of Embryology and Experimental Morphology 51: 51-62, 1979

Tsukushi cooperates with VG1 to induce primitive streak and Hensen's node formation in the chick embryo. Development 133(19): 3777-3786, 2006

Researches on the formation of axial organs in the chick embryo. XI. Experimental investigations into the role of Hensen's node in somitogenesis. Morphologie et Embryologie 30(1): 3-10, 1984

Tracking and ablating subpopulations of epiblast cells in the chick embryo. Biological Procedures Online 10: 74-82, 2008

Commitment of mesoderm cells in hensen's node of the chick embryo to notochord and somite. Development (Cambridge) 114(2): 403-415, 1992

Subpopulations of NeuroM and MyoD positive cells in the chick embryo epiblast. FASEB Journal 17(4-5): Abstract No 467 8, 2003

N-cadherin promotes the differentiation of chick embryo epiblast cells into skeletal muscle. Molecular Biology of the Cell 7(SUPPL ): 40A, 1996

Variability in the intercellular junction of epiblast cells in relation with the position of them in chick embryo. Zoological Science 8(6): 1099, 1991

Skeletal myogenesis: the preferred pathway of chick embryo epiblast cells in vitro. Developmental Biology 173(1): 279-291, 1996

Chemotaxis of the pre-streak stage epiblast cells to the posterior marginal zone in the chick embryo. Zoological Science (Tokyo) 10(SUPPL ): 49, 1993

Autoradiographic study of the fate of cells invaginating through Hensen's node in the chick embryo at the definitive streak stage. Acta Embryologiae et Morphologiae Experimentalis 8(3): 213-220, 1965

Developmental potential of chick embryo epiblast cells Differentiation in the absence of gastrulation and tissue interactions. Molecular Biology of the Cell 5(SUPPL ): 352A, 1994

Developmental potential of chick embryo epiblast cells Skeletal myogenesis in the absence of exogenous proteins and replication. Molecular Biology of the Cell 5(SUPPL ): 162A, 1994