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Toxicological application of luminol dependent chemiluminescence assay in hepatic microsomes and reconstituted cytochrome p450 enzyme system



Toxicological application of luminol dependent chemiluminescence assay in hepatic microsomes and reconstituted cytochrome p450 enzyme system



Zhongguo Yaolixue Yu Dulixue Zazhi 5(4): 312-320



A mechanism of luminol-dependent chemiluminescence in microsomal cytochrome P450 enzymes has been studied. The results indicated that the chemiluminescence depended not only on NADPH induction but also on intact cytochrome P450 enzymes, and may be used as an assay of catalytic activity of the enzymes. The study of chemiluminescence in detecting free radicals formed by biotransformation of 44 xenobiotics suggested that some of the compounds known as free radical producing agents by bioactivation increased the light emission in the cytochrome P450 enzyme system, such as carbon tetrachloride, chloroform, tetrachloroethylene, etc., others forming toxic metabolites by biotransformation induced chemiluminescence in some cases, such as carbon disulfide, benzene, methyldurshan, methylparathion, etc. or decreased the light emission, such as parathion, aniline and polychlorinated biphenyls etc., and still others whose toxic actions have no relationship with their biotransformation had no influence or an inhibitory effect on the chemiluminescence, such as aminopyrine, TOCP, and acrylamide. Chemiluminescence in the cytochrome P450 enzyme system remained unchanged during the bioactivation of carcinogens such as diethylnitrosamine, dipropylnitrosamine and benzo(a)pyrene as compared with the control.

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