Section 8
Chapter 7,960

Trans effect of cellular potassium and chloride on ouabain resistant rubidium potassium influx in low potassium sheep red bleed cells further evidence for asymmetry of potassium chloride cotransport

Delpire, E.; Lauf, P.K.

Pfluegers Archiv European Journal of Physiology 419(5): 540-542


Accession: 007959193

The electroneutral K-Cl cotransport in low K+ (LK) sheep red blood cells is kinetically asymmetric and thermodynamically outward poised (Delpire and Lauf, 1991a). We have shown previously by trans-inhibition kinetics that Cl- binds prior to K+ to the outside configuration of the carrier. In the present study, we confirm that K+ and Cl- bind randomly to the cytoplasmic aspect of the transporter because K+ in the absence of Cl-, and Cl- in the absence of K+, trans-inhibit ouabain-resistant Rb+ (K+) influx in these cells. In contrast to the trans-inhibition pattern observed outside, neither K+ nor Cl- trans-inhibit K+(Rb+) influx in the presence of the cotransported ion, further supporting the assymetry of the K-Cl cotransporter.

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