+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Ultrastructural and immunocytochemical studies on the cytoskeleton in the anterior pituitary of rats with special regard to the relationship between actin filaments and secretory granules



Ultrastructural and immunocytochemical studies on the cytoskeleton in the anterior pituitary of rats with special regard to the relationship between actin filaments and secretory granules



Cell & Tissue Research 258(1): 25-30



As previously reported, in anterior pituitary cells of the rat, secretory granules are linked with adjacent granules, cytoorganelles, microtubules, and plasma membrane by thin filaments, 4-10 nm in diameter. The quick-freeze, deep-etching method revealed that some of the filaments linking adjacent secretory granules show 5 nm-spaced striations on their surface which are know to be characteristic of actin. Immunocytochemistry showed that actin is localized in the cytoplasm beneath the plasma membrane, and around or between secretory granules. The heavy meromyosion decoratin method demonstrated that actin filaments are mainly located in the cytoplasm beneath the plasma membrane, while some actin filaments are connected with the limiting membrane of the secretory granules. The actin filaments associated with the secretory granules are considered to be involved in the intracellular transport of the granules, while those localized in the peripheral cytoplasmic matrix might control the approach of the secretory granules to the plasma membrane and their release.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 007978501

Download citation: RISBibTeXText

PMID: 2680099

DOI: 10.1007/bf00223140


Related references

Muscle actin filaments bind pituitary secretory granules in vitro. Journal of Cell Biology 73(1): 78-87, 1977

Ultrastructural studies on dynamic changes of golgi area and secretory granules in pituitary mt cells of hypothalamic lactating rats. Acta Histochemica et Cytochemica 8(1): 73, 1975

Ultrastructural aspects of cytoskeleton in anterior pituitary cells and its possible function on the secretory activity. Cell Structure & Function 13(6): 593, 1989

Isolation and biological properties of secretory granules from rat anterior pituitary granules from rat anterior pituitary glands. Journal of Cell Biology 43: 564-574, 1969

Quantitative analysis of anterior pituitary cells and their secretory granules from rats during estrous cycle. Igaku Kenkyu. Acta Medica 47(8): 509-520, 1977

Actin filaments and intracytoplasmic movement of secretory granules. Journal of Dental Research 68(SPEC ISSUE JUNE): 956, 1989

Colocalization of galanin and prolactin within secretory granules of anterior pituitary cells in estrogen-treated Fischer 344 rats. Endocrinology 129(1): 270-276, 1991

An ultrastructural immunocytochemical study of secretory granules of an adenohypophyseal tumor. Bollettino Della Societa Italiana di Biologia Sperimentale 58(21): 1394-1399, 1982

Sorting of three secretory proteins to distinct secretory granules in acidophilic cells of cow anterior pituitary. Journal of Cell Biology 105(4): 1579-1586, 1987

Ultrastructural observation of the trans-Golgi associated plate-like cisterna in the secretory cells of the rat anterior pituitary gland with special reference to the intracisternal skeleton. Anatomical Record 225(4): 272-278, 1989

Actin cytoskeleton remodelling in the anterior pituitary folliculostellate cell line TtT/GF: participation of the actin-binding protein cortactin. Journal of Molecular Histology 36(8-9): 461-474, 2005

Cytological, immunocytochemical and ultrastructural studies of the anterior lobe of the pituitary gland in goat kids: gonadotrophic cells. Anales de Veterinaria de Murcia 3: 29-42, 1987

Organization of actin filaments in early chick embryo ectoderm: an ultrastructural and immunocytochemical study. Journal of Embryology and Experimental Morphology 69: 169-182, 1982

Cytological, immunocytochemical and ultrastructural studies of the anterior lobe of the pituitary gland of goat kids (Capra hircus): ACTH cells. Anales de Veterinaria de Murcia 3: 17-27, 1987

Secretory granules from anterior pituitary glands. Heller, H And K Lederis (Edited By) Memoirs Of The Society For Endocrinology, No 19 Subcellular Organization And Function in Endocrine Tissues Symposium Xxiii + 1012p Illus Cambridge University Press: New York, N Y , U S A 161-184, 1971