+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Ultrastructural localization of synaptophysin to the secretory granules of normal glucagon and insulin cells in human islets of Langerhans



Ultrastructural localization of synaptophysin to the secretory granules of normal glucagon and insulin cells in human islets of Langerhans



Ultrastructural Pathology 15(3): 215-219



Immunogold labeling of the pancreatic islets in humans by means of monoclonal antibodies to synaptophysin resulted in a distinct localization of gold particles to the secretory granules of glucagon-immunoreactive cells. The same type of immunoreactivity cells. The same type of immunoreactivity was noted with antiserum to chromogranin A. Glucagon immunoreactivity was concentrated in the dense central core of the secretory granules. Some immunoreactivity for synaptophysin was also found in the secretory granules of the insulin-producing cells, although it was weaker in this location.

Accession: 007979383

Download citation: RISBibTeXText

PMID: 1908157

DOI: 10.3109/01913129109021883


Related references

Ultrastructural Localization of Insulin-like Growth Factor-2 (Igf-2) to the Secretory Granules of Insulin Cells: A Study in Normal and Diabetic (Gk) Rats. Ultrastructural Pathology 21(5): 457-466, 1997

Ultrastructural localization of insulin-like growth factor-2 to the secretory granules of insulin cells A study in normal and diabetic rats. Ultrastructural Pathology 21(5): 457-466, 1997

Vesicular Inhibitory Amino Acid Transporter Is Present in Glucagon-Containing Secretory Granules in Tc6 Cells, Mouse Clonal -Cells, and -Cells of Islets of Langerhans. Diabetes 52(8): 2066-2074, 2003

PACAP is expressed in secretory granules of insulin and glucagon cells in human and rodent pancreas: Evidence for generation of cAMP compartments uncoupled from hormone release in diabetic islets. Regulatory Peptides 113(1-3): 31-39, 2003

Vesicular inhibitory amino acid transporter is present in glucagon-containing secretory granules in alphaTC6 cells, mouse clonal alpha-cells, and alpha-cells of islets of Langerhans. Diabetes 52(8): 2066-2074, 2003

Co-localization of islet amyloid polypeptide and insulin in the B cell secretory granules of the human pancreatic islets. Diabetologia 32(4): 240-244, 1989

A small-scale method for the isolation of insulin-containing secretory granules from islets of Langerhans. Analytical Biochemistry 166(1): 142-149, 1987

Localization of inositol trisphosphate receptor subtype 3 to insulin and somatostatin secretory granules and regulation of expression in islets and insulinoma cells. Proceedings of the National Academy of Sciences of the United States of America 91(16): 7777-7781, 1994

Plasminogen activator in the islets of langerhans possible localization in the insulin containing granules. Journal of Cell Biology 91(2 Part 2): 223A, 1981

Effects of osmolarity and anion substitution on insulin release from rat islets of langerhans and isolated secretory granules. Diabetes Research and Clinical Practice 1: S275, 1985

Type IB secretory phospholipase A2 is contained in insulin secretory granules of pancreatic islet beta-cells and is co-secreted with insulin from glucose-stimulated islets. Biochimica et Biophysica Acta 1390(3): 301-312, 1998

Ultrastructural identification of diabetes associated peptide islet amyloid polypeptide in secretory granules and lysosomes of b cells in human and monkey pancreatic islets. Diabetologia 31(7): 480A, 1988

Electron microscopic studies on the secretory mechanism of pancreatic islet cells, with particular reference to beta cells. II. Secretion of beta granules in islets of Langerhans, particularly in association with intracellular reactive zinc under normal conditions, during prolonged starvation, and after administration of dithizone in rabbits. Endocrinologia Japonica 13(4): 384-408, 1966

Ultrastructural localization of osteopontin immunoreactivity in phagolysosomes and secretory granules of cells in human intestine. Histochemical Journal 29(11-12): 801-812, 1997

Localization of islet amyloid peptide in lipofuscin bodies and secretory granules of human b cells and in islets of type 2 diabetic subjects. Cell and Tissue Research 257(1): 179-186, 1989