Variation in high molecular weight glutenin subunits of triticum aestivum and triticum turgidum ssp dicoccoides

Kolster, P.; Krechting, C.F.; Van Gelder, W.M.J.

Euphytica (Suppl): 141-146


ISSN/ISBN: 0014-2336
Accession: 007994519

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Two-dimensional electrophoresis revealed biochemical variation for glu-B1 high molecular weight (HMW) glutenin subunit 7 present in Dutch wheat (Triticum aestivum) varieties. This variation was not recognized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). In T. turgidum ssp. dicoccoides, HMW glutenin subunits were detected which do not occur in varieties. Some of these subunits show similar relative mobilities during SDS-PAGE as subunits present in varieties and as such unambiguous identification is not possible. Before species related T. aestivum are used in a breeding programme, their HMW glutenin subunits should be identified by two-dimensional electrophoresis, in order to avoid the introduction of HMW glutenin subunits, of which the influence on bread-making quality is not known.