Variations in glomerular basement membrane polyanion in adriamycin induced nephrotic rats antiplatelet agent administration
Namba, S.; Kimura, K.; Tojo, A.; Matsuoka, H.; Sugimoto, T.
Japanese Journal of Clinical Electron Microscopy 24(2): 179-186
To investigate the relationship between platelet aggregation and glomerular basement membrane (GBM) polyanion in adriamycin (ADR) nephrosis in rats, an intervention study with 3 antiplatelet agents was performed. After ADR injection, 48 rats were divided into 4 groups and orally administered the agents or vehicle, dipyridamole (ADR-DI), ticlopidine (ADR-TI), trapidil (ADR-TR) and distilled water (ADR) every day for 8 weeks. As a control, 10 rats were injected isotonic saline and maintained wih distilled water. After 10 weeks, urinary protein excretions were significantly lessened in the ADR-DI group and platelet aggregation was significantly suppressed in both the ADR-TI and ADR-TR compared to ADR. GBM anion in lamina rara externa (LRE) was reduced in the 4 ADR-treated groups compared to the control, and preserved in the ADR-DI group compared to the ADR group. These results show that there appears to be no significant correlation between suppression of platelet aggregation and attenuation of renal damages, and that polyanions in LRE may contribute to prevention of protein loss from GBM.