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Volume regulatory potassium fluxes in the isolated perfused rat liver characterization by ion transport inhibitors

Volume regulatory potassium fluxes in the isolated perfused rat liver characterization by ion transport inhibitors

American Journal of Physiology 257(3 Part 1): G357-G363

ISSN/ISBN: 0363-6143

Net hepatic release and uptake of K+ were examined in isolated perfused rat livers subjected to a 10-min period of hypotonic stress. Effluent Na+, K+, and Ca2+ activities were monitored throughout. Initiation and termination of hypotonic stress triggered sharp transient (< 1 min) changes in effluent ion activities that indicated net water movement into and out of the liver, respectively. In addition, hypotonic stress caused a large transient net release of hepatic K+, whereas return to isotonicity triggered a transient net hepatic K+ uptake. The hypotonically induced K+ release was inhibited by 2 mM barium (95%) and by 1 mM quinine (60%). Net K+ influx, on th other hand, was inhibited by 1 mM ouabain (100%) and by 1 mM amiloride (50%). Osmotically induced K+ fluxes were not significantly affected by bicarbonate removal and were only partially inhibited by 0.1 mM 4,4'-diisothiocyanostilbene-2,2' -disulfonic acid (DIDS) or bumetanide. The results suggest that K+ conductance increases during hypotonic stress, whereas return to isotonicity induces a ouabain-sensitive K+ uptake partly because of increased Na+-H+ exchange. These mechanisms probably participate in regulatory volume decrease and regulatory volume increase, respectively.

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Accession: 008002652

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