+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Zinc inhibition of glucose uptake in brush border membrane vesicles from pig small intestine



Zinc inhibition of glucose uptake in brush border membrane vesicles from pig small intestine



Pfluegers Archiv European Journal of Physiology 415(2): 165-171



The effect of zinc on sodium coupled glucose uptake was studied in pig intestinal brush border membrane vesicles. In this system zinc inhibited glucose uptake and appeared to have a Ki of 0.25 mM. When tested by spectrophotometry, electron microscopy and protein determination following centrifugation, no evidence of significant vesicle aggregation was found with 0.5 mM zinc treatment. Zinc inhibition of glucose uptake persisted when the vesicle membrane potential was clamped with identical KCl concentrations inside and outside the vesicles in the presence of valinomycin. Variation of the glucose and sodium concentrations gave results indicating that zinc reduces glucose affinity for the carrier but not sodium binding to the transporter. The glucose inhibitory effect was not due to a rapid dissipation of the sodium gradient as zinc failed to affect sodium uptake in the absence of glucose. Zinc also failed to inhibit glucose efflux from vesicles under isotopic exchange conditions, when glucose and sodium concentrations were identical inside and outside vesicles. T t1/2 of glucose inhibition by zinc was relatively long, i.e. 6 min. We conclude that zinc acts as an inhibitor of glucose transport by interacting with the sodium-glucose co-transporter. The long zinc incubation time required to achieve maximal inhibition of glucose transport suggests that this interaction takes place.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008009573

Download citation: RISBibTeXText

PMID: 2594473

DOI: 10.1007/bf00370588


Related references

Zinc uptake into brush border membrane vesicles from pig small intestine. Leger, C L And G Bereziat (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 195 Biomembranes Et Nutrition: Nutriments Affectant La Composition Lipidique Et Les Proprietes Des Membranes Cellulaires; (Inserm (National Institute Of Health And Medical Research) Colloquium, Vol 195 Biomembranes And Nutrition: Nutrients Affecting Lipid Composition And Properties Of Cell Membranes); International Meeting, Paris, France, June 12-14, Xix+575p Les, 1989

Zinc uptake by brush border membrane vesicles (BBMV) from pre-weanling rat small intestine. Trace elements in man and animals 6 edited by Lucille S Hurley et al: 600, 1988

Effect of calcium on the zinc uptake by brush border membrane vesicles isolated from the rat small intestine. Agricultural and Biological Chemistry 55(11): 2813-2816, 1991

Zinc inhibition of sodium stimulated glucose uptake by intestinal brush border membrane vesicles. Federation Proceedings: 1081, 1987

Changes in glucose uptake by and phlorizin binding to brush-border membrane vesicles of small intestine from streptozotocin-induced diabetic rats. Journal of Nutritional Science and Vitaminology 34(3): 327-334, 1988

Manganese uptake by brush border membrane vesicles from rat small intestine. Federation Proceedings 45(3): 368, 1986

Uptake of methylchlorpromazine by brush-border membrane vesicles from rat small intestine. Biological and Pharmaceutical Bulletin 20(6): 662-666, 1997

Mechanisms of zinc transport into pig small intestine brush-border membrane vesicles. Journal of Physiology 465: 57-72, 1993

Some aspects of zinc transport into pig small intestine brush border membrane vesicles. Biophysical Journal 64(2 Part 2): A82, 1993

Changes in D-glucose uptake by brush-border vesicles from small intestine of rats treated with mitomycin C. Biochemical Pharmacology 35(7): 1153-1158, 1986

Studies of zinc transport into brush-border membrane vesicles isolated from pig small intestine. Biochimica et Biophysica Acta 1024(2): 323-330, 1990

L-Threonine uptake into brush border membrane vesicles of different segments of porcine small intestine. Digestive physiology in pigs Proceedings of the 7th international symposium, Saint Malo, France 26-28 May 1997: 53-56, 1997

Characterization of calcium uptake by brush border membrane vesicles of human small intestine. Gastroenterology 96(1): 122-129, 1989

L amino acid uptake by brush border membrane vesicles from bovine small intestine. Journal of Dairy Science 68(Suppl. 1): 173, 1985

Kinetics of glutamate transport in brush border membrane vesicles from rat intestine inhibition by glucose. Bulletin of Molecular Biology and Medicine 13(1-4): 13-24, 1988