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3-beta-Chlorosteroids-nutritonal and toxicological effects in mice


, : 3-beta-Chlorosteroids-nutritonal and toxicological effects in mice. Fett Wissenschaft Technologie 97(5): 183-189

3-Chlorosteroids, e.g. 3-chlorocholest-5-ene and 3-chlorostigmast-5-ene, are formed from the corresponding sterols during the industrial hydrolysis of proteins for the production of flavor enhancers. In future, these compounds may also attract attention as environmental contaminants. A simple method for the fractionation and quantitative determination of 3-beta-chlorosteroids by C-18 reversed-phase HPLC has been developed and used for the analysis of these chlorinated steroids in protein hydrolysates. In addition, the biological effects of orally administrated 3-beta-chloro-steroids were tested in mice that were fed 3-beta-chlorocholest-5-ene and 3-beta-chlorostigmast-5-ene in doses of 1 and 10 mg/animal/day. The results of our study showed that body and organ weights as well as feed intake of the various experimental groups were not significantly different from those of the control group. The quantitative determination of 3-beta-chlorosteroids in organs and tissues revealed that intestinal absorption of 3-beta-chlorocholest-5-ene was higher than that of, 3-beta-chlorostigmast-5-ene. Highest concentration of 3-beta-chlorocholest-5-ene was detected in liver and adipose tissue. Absorption and distribution in tissues as well as metabolism of both radioactive 3-beta-chloro-(4-14C)cholest-5-ene and 3-beta-chloro-(4-14C)stigmast-5-ene have also been studied in mice. At 2 hours after stomach incubation of the two substrates, large proportions of radioactivity had passed through the small intestine and were concentrated in the contents of caecum and colon. Only small proportions of radioactivity were detected outside the alimentary canal, predominantly in the liver. Histopathological examination of sections of organs and tissues such as stomach, duodenum, liver, kidneys, and spleen showed no indications of irreversible cell damages caused by 3-beta-chlorosteroids.

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