A chromosome painting method for human sperm chromosomes using fluorescent in situ hybridization

Cui, X.; Tateno, H.; Hayata, I.; Sato, K.; Kamiguchi, Y.

Japanese Journal of Human Genetics 39(2): 255-258

1994


ISSN/ISBN: 0916-8478
PMID: 8086643
DOI: 10.1007/bf01876846
Accession: 008027592

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Abstract
A method of chromosome painting on human sperm chromosomes using fluorescent in situ hybridization (FISH) is introduced. Sperm chromosome slides were prepared after in vitro fertilization of hamster eggs with human spermatozoa. The slides were treated by RNase A before FISH. Chromosome 4 was clearly and specifically painted in a majority of sperm-derived metaphase plates after an application of whole chromosome painting DNA probes of this chromosome. This is the first report of successful painting on human sperm chromosomes.

A chromosome painting method for human sperm chromosomes using fluorescent in situ hybridization