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Chapter 8,039

A fluorometric assay of ceramide glycanase with 4-methylumbelliferyl beta-D-lactoside derivatives

Wang, L.X.; Pavlova, N.V.; Li, S.C.; Li, Y.T.; Lee, Y.C.

Glycoconjugate Journal 13(3): 359-365

1996

ISSN/ISBN: 0282-0080
PMID: 8781966
DOI: 10.1007/bf00731468
Accession: 008038886
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4-Methylumbelliferyl 6'-O-benzyl-beta-D-lactoside (6'Bn-MU-Lac) and some related compounds were synthesized via different selective reactions including phase-transfer glycosylation. Their suitability as substrates for a fluorometric assay of ceramide glycanase (CGase) was evaluated. Among others, the 6'Bn-MU-Lac, which is resistant to exogalactosidase, was found to be a suitable substrate for routine assay of the CGase activity. For American leech CGase, the K(m) value is 0.232 mM at pH 5.

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