+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana



A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana



Proceedings of the National Academy of Sciences of the United States of America 92(9): 3903-3907



A cDNA corresponding to a putative phosphatidylinositol-specific phospholipase C (PI-PLC) in the higher plant Arabidopsis thaliana was cloned by use of the polymerase chain reaction. The cDNA, designated cAtPLC1, encodes a putative polypeptide of 561 aa with a calculated molecular mass of 64 kDa. The putative product includes so-called X and Y domains found in all PI-PLCs identified to date. In mammalian cells, there are three types of PI-PLC, PLC-beta, -gamma, and -delta. The overall structure of the putative AtPLC1 protein is most similar to that of PLC-alpha, although the AtPLC1 protein is much smaller than PLCs from other organisms. The recombinant AtPLC1 protein synthesized in Escherichia coli was able to hydrolyze phosphatidylinositol 4,5-bisphosphate and this activity was completely dependent on Ca2+, as observed also for mammalian PI-PLCs. These results suggest that the AtPLC1 gene encodes a genuine PI-PLC of a higher plant. Northern blot analysis showed that the AtPLC1 gene is expressed at very low levels in the plant under normal conditions but is induced to a significant extent under various environmental stresses, such as dehydration, salinity, and low temperature. These observations suggest that AtPLC1 might be involved in the signal-transduction pathways of environmental stresses and that an increase in the level of AtPLC1 might amplify the signal, in a manner that contributes to the adaptation of the plant to these stresses.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008039729

Download citation: RISBibTeXText

PMID: 7732004

DOI: 10.2307/2367455


Related references

A gene encoding phosphatidylinositol-4-phosphate 5-kinase is induced by water stress and abscisic acid in Arabidopsis thaliana. Plant Journal 15(4): 563-568, 1998

AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thaliana. Plant molecular biology 34(1): 175-180, 1997

The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana. M G G: Molecular and general genetics 238(1-2): 17-25, 1993

Overexpression of Arabidopsis thaliana LTL1, a salt-induced gene encoding a GDSL-motif lipase, increases salt tolerance in yeast and transgenic plants. Plant Cell and Environment 29(10): 1890-1900, 2006

A novel Glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic Arabidopsis thaliana. Journal of Plant Physiology 168(11): 1241-1248, 2011

Identification of a Receptor-like Protein Kinase Gene Rapidly Induced by Abscisic Acid, Dehydration, High Salt, and Cold Treatments in Arabidopsis thaliana. Plant Physiology 113(4): 1203-1212, 1997

Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt, and cold treatments in Arabidopsis thaliana. Plant Physiology 113(4): 1203-1212, 1997

Ectopic overexpression of a novel Glycine soja stress-induced plasma membrane intrinsic protein increases sensitivity to salt and dehydration in transgenic Arabidopsis thaliana plants. Journal of Plant Research 128(1): 103-113, 2015

ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana. Plant Journal 56(3): 411-422, 2008

Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A. Febs Letters 586(4): 373-377, 2012

Cis-regulatory elements of the peroxidase gene in Arabidopsis thaliana involved in root-specific expression and responsiveness to high-salt stress. Annals of the new York Academy of Sciences 782: 107-114, 1996

Maize similar to RCD1 gene induced by salt enhances Arabidopsis thaliana abiotic stress resistance. Biochemical and Biophysical Research Communications 503(4): 2625-2632, 2018

Effect of salt stress on genes encoding translation-associated proteins in Arabidopsis thaliana. Plant Signaling and Behavior 7(9): 1095-1102, 2012

Differential mRNA translation contributes to gene regulation under non-stress and dehydration stress conditions in Arabidopsis thaliana. Plant Journal 38(5): 823-839, 2004

Identification of a gene family encoding dehydration-responsive-element binding proteins in Arabidopsis thaliana and analysis of its promoter. Plant and Cell Physiology 39(Suppl. ): S106, 1998