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A high performance liquid chromatography method for quantification of DIBOA, DIMBOA, and MBOA from aqueous extracts of corn and winter cereal plants



A high performance liquid chromatography method for quantification of DIBOA, DIMBOA, and MBOA from aqueous extracts of corn and winter cereal plants



Journal of Liquid Chromatography 17(12): 2651-2665



A new HPLC method is described for the quantification of aglycones derived from cyclic hydroxamic acids: DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one), its degradation product MBOA (6-methoxybenzoxazolinone) and the demethoxy analogue DIBOA (2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one) in crude aqueous solutions from homogenized cereal tissues. After hydrolysis of beta-glycosides to aglycones, extracts of cereal samples were chromatographed on a PRP-1 column using a gradient of tris/citric acid and methanol/acetonitrile, and monitored at 288 nm. The three compounds were separated within 26 min. The minimum detection limit for all of them was 50 pmol. The recovery percentage was 100% for DIBOA, 87% for DIMBOA and 96% for MBOA. The levels of aglycones were determined in aqueous extracts from corn and winter cereal seedlings (20-100 mg), ranging from 3.80 to 11.50 mg DIBOA/g dry weight and 4.75 to 100.43 mg DIMBOA/g dry weight.

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Accession: 008041213

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DOI: 10.1080/10826079408013405



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