+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A new mouse model for embryos with a hatching deficiency and its use to elucidate the mechanism of blastocyst hatching

A new mouse model for embryos with a hatching deficiency and its use to elucidate the mechanism of blastocyst hatching

Fertility and Sterility 59(6): 1296-1301

Objective: To develop an experimental model for embryos with a defect specific to hatching, with the purpose of clarifying the mammalian embryo hatching mechanism. Design: A microneedle was inserted under the zona pellucida (ZP) of mouse embryos, and either 1/2 or 1/4 of the blastomeres were mechanically destroyed. The remainder of embryos that developed into blastocysts were compared for hatching to controls wherein a microneedle was inserted and withdrawn without harming the embryo. Experiments were done to increase pressure within the perivitelline space to decrease the amount of ZP material to study the hatching mechanism. Setting: University-based basic research laboratory. Results: When 1/2 or 1/4 of the embryo was destroyed and the remaining cells developed into healthy blastocysts within the intact zona, hatching was significantly impaired, and zona thickness was markedly increased relative to controls. When a mineral oil droplet was inserted under the zona to enhance a possible mechanical component of hatching, manipulated embryos were not restored to normal hatching, and hatching of unmanipulated blastocysts was not improved. However, when the zona was circumferentially thinned by application of acid Tyrode's solution, the hatching defect in manipulated "3/4 embryos" was corrected. Conclusion: Because it is known that embryos with cells reduced by 1/2 or 1/4 have normal developmental potential, we conclude that the inability of such embryos to hatch from an intact zona constitutes a mouse model for a defect specific to hatching. Moreover, results from mineral oil droplet insertion and circumferential thinning of the zona indicate that normal hatching is accomplished predominantly, if not entirely, by zona lysis, not by pressure exerted against the zona by the expanding blastocyst.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008053877

Download citation: RISBibTeXText

PMID: 8495780

Related references

Comparison of laser-assisted hatching and acidified Tyrode's hatching by evaluation of blastocyst development rates in sibling embryos: a prospective randomized trial. Fertility and Sterility 85(2): 487-491, 2006

Trypsin-like Hatching Enzyme of Mouse Blastocysts: Evidence for Its Participation in Hatching Process before Zona Shedding of Embryos : (embryo/hatching enzyme/protease/trypsin/strypsin). Development, Growth & Differentiation 36(2): 149-154, 1994

Blastocyst formation and hatching in vitro following zona drilling of mouse and human embryos. Gamete Research 24(1): 67-80, 1989

Period of action of mouse blastocyst hatching enzyme and its histochemical localization in embryos. Zoological Science 4(6): 1044, 1987

Improvement in blastocyst hatching of mouse embryos cocultured with human placental cells. Journal Of Assisted Reproduction & Genetics. 10(7): 463-467, 1993

Effects of removal of necrotic blastomeres from mouse cryopreserved embryos on blastocyst formation and hatching. Theriogenology 64(5): 1114-1120, 2005

Restoration of normal implantation rates in mouse embryos with a hatching impairment by use of a new method of assisted hatching. Fertility and Sterility 59(6): 1302-1307, 1993

Hatching of an estuarine crab, Sesarma haematocheir: factors affecting the timing of hatching in detached embryos, and enhancement of hatching synchrony by the female. Journal of Oceanography. February; 561: 93-102, 2000

Hatching of an Estuarine Crab, Sesarma Haematochier: Factors Affecting the Timing of Hatching in Detached Embryos, and Enhancement of Hatching Synchrony by the Female. Journal of Oceanography 56(1): 93-102, 2000

Trypsin-like hatching enzyme of mouse blastocysts: Evidence for its participation in hatching process before zona shedding of embryos. Development Growth & Differentiation 36(2): 149-154, 1994

High survival and hatching rates following vitrification of embryos at blastocyst stage: a bovine model study. Reproductive Biomedicine Online 14(4): 464-470, 2007

Effects of blastocyst artificial collapse prior to vitrification on hatching and survival rates and the expression of klf4 gene in mouse embryos. Veterinary Research Forum 9(1): 87-92, 2018

Physiological characterization of blastocyst hatching mechanims by use of a mouse antihatching model. Fertility & Sterility 63(2): 288-294, 1995

The use of Matrigel at low concentration enhances in vitro blastocyst formation and hatching in a mouse embryo model. Fertility & Sterility 71(6): 1133-1137, 1999

The in vitro development of mouse embryos beyond the blastocyst stage into the hatching and outgrowth stage using different energy sources. Journal of Assisted Reproduction & Genetics 13(10): 786-792, 1996