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A nicotinic acetylcholine receptor subunit from insect brain forms a non-desensitising homo-oligomeric nicotinic acetylcholine receptor when expressed in Xenopus oocytes


A nicotinic acetylcholine receptor subunit from insect brain forms a non-desensitising homo-oligomeric nicotinic acetylcholine receptor when expressed in Xenopus oocytes



Neuroscience Letters 199(2): 107-110



ISSN/ISBN: 0304-3940

PMID: 8584235

DOI: 10.1016/0304-3940(95)12033-z

The locust alpha-like nicotinic receptor subunit alpha-L1 was expressed in Xenopus oocytes. Small but reproducible currents were elicited by application of high concentrations of nicotine, demonstrating that alpha-L1 is capable of forming homo-oligomeric channels. Nicotine-evoked currents were blocked by alpha-bungarotoxin and methyllycaconitine. Comparison with chick alpha-7 receptors showed that the two receptors differ with respect to nicotine sensitivity and time course of evoked currents. Nicotine dose-response curves gave EC-50 values of 24 and 830 mu-M for alpha-7 and alpha-L1 respectively. Whereas alpha-7 responses showed characteristic fast onset and rapid desensitization within 3 s, alpha-L1 currents displayed a slow onset and showed no tendency to desensitize during 45 s of agonist application. Thus alpha-L1 is a novel nicotinic subunit for the further exploration of structure-function relationships of ligand-gated ion channels. The question of the subunit composition of native insect receptors remains open.

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Accession: 008057978

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