A novel method for chemical modification of functional groups other than a carboxyl group in proteins by N-ethyl-5-phenylisooxazolium-3'-sulfonate (Woodward's reagent-K) : inhibition of ADP-induced platelet responses involves covalent modification of aggregin, an ADP receptor
Puri, R.N.; Colman, R.W.
Analytical Biochemistry 240(2): 251-261
1996
ISSN/ISBN: 0003-2697 PMID: 8811919 DOI: 10.1006/abio.1996.0355
Accession: 008060472
The chemical reaction of N-ethyl-5-phenylisooxazolium-3'-sulfonate (Woodward's Reagent-K, WR-K) with a carboxyl group yields an enol ester that cannot be reduced by sodium borohydride in an aqueous solution, while other nucleophiles such as sulfhydryl, hydroxyl, amino, and imidazole groups, react with WR-K to yield unsaturated ketones that are capable of being reduced by sodium borohydride in an aqueous medium.