+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Amplification of TCRbeta gene rearrangements from micromanipulated single cells: T cells rosetting around Hodgkin and Reed-Sternberg cells in Hodgkin's disease are polyclonal



Amplification of TCRbeta gene rearrangements from micromanipulated single cells: T cells rosetting around Hodgkin and Reed-Sternberg cells in Hodgkin's disease are polyclonal



European Journal of Immunology 28(8): 2424-2431



Despite accounting for only a minor fraction of all cells in Hodgkin's lymphoma tissue, the Hodgkin and Reed-Sternberg (HRS) cells represent the malignant tumor cell clone in Hodgkin's disease (HD). By far the most abundant cell type in the tumor tissue are CD4+ T cells. Some of them intimately associate with HRS cells forming rosettes around them. This study addresses the question whether the rosetting phenomenon reflects a specific interaction between T and HRS cells by asking whether the rosettes are composed of T cells expressing a restricted TCR repertoire. Single rosetting T cells were micromanipulated from frozen sections of tumor tissue in two cases of nodular sclerosing HD and one case of lymphocyte predominant HD. TCR Vbeta gene rearrangements were amplified from these single cells by PCR. Of 83 potentially functional Vbeta gene rearrangements obtained altogether from the three cases, 81 were found to be clonally unrelated. Furthermore, they did not show signs of selection of the receptor chains for recognition of common epitopes: The usage of Vbeta and Jbeta gene segments as well as the distribution of complementarily-determining region (CDR) 3 lengths was similar to what was seen in a collection of 60 Vbeta gene rearrangements from blood of healthy donors and no recurrent CDR3 amino acid motifs were found. These data suggest that the HRS cells attract CD4+ T cells nonspecifically.

(PDF emailed within 1 workday: $29.90)

Accession: 008137185

Download citation: RISBibTeXText

PMID: 9710220


Related references

Cytokine gene expression in Hodgkin and Reed-Sternberg cells of Hodgkins disease by single-cell global PCR IL-1 and TNF-alpha but not IL-2, IL-4 and IFN-gamma are produced by Hodgkin and Reed-Sternberg cells. Blood 86(10 SUPPL 1): 179A, 1995

Hodgkin's disease: immunoglobulin heavy and light chain gene rearrangements revealed in single Hodgkin/Reed-Sternberg cells. Molecular Pathology 52(1): 37-41, 1999

An immunohistochemical analysis of Reed-Sternberg-like cells in posttransplantation lymphoproliferative disorders: The possible pathogenetic relationship to Reed-Sternberg cells in Hodgkin's disease and Reed-Sternberg-like cells in non-Hodgkin's lymphomas and reactive conditions. Human Pathology 28(4): 493-498, 1997

Hodgkin disease: Hodgkin and Reed-Sternberg cells picked from histological sections show clonal immunoglobulin gene rearrangements and appear to be derived from B cells at various stages of development. Proceedings of the National Academy of Sciences of the United States of America 91(23): 10962-6, 1994

Hodgkin's disease: clonal Ig gene rearrangements in Hodgkin and Reed-Sternberg cells picked from histological sections. Annals of the New York Academy of Sciences 764: 523-524, 1995

Hodgkin and Reed-Stenberg-like cells in B-cell chronic lymphocytic leukemia represent the outgrowth of single germinal-center B-cell-derived clones: Potential precursors of Hodgkin and Reed-Sternberg cells in Hodgkin's disease. Blood 95(3): 1023-1031, Feb 1, 2000

Interleukin-13-receptor cDNA is expressed in single Hodgkin and Reed-Sternberg cells of Hodgkins disease Possibility of an autocrine loop in Hodgkin cells. Onkologie 23(Sonderheft 7): 144, 2000

Hodgkin and Reed-Sternberg cell-associated autoantigen CLIP-170/restin is a marker for dendritic cells and is involved in the trafficking of macropinosomes to the cytoskeleton, supporting a function-based concept of Hodgkin and Reed-Sternberg cells. Blood 100(12): 4139-4145, 2002

Are there cases of Hodgkin's disease with polyclonal Reed Sternberg cells?. Pathologica 88(2): 147-148, 1996

Amplification of the MDM2 and lack of mutations of the p53 gene in Hodgkin and Reed Sternberg cells of Hodgkins disease Results from single cell polymerase chain reaction and molecular cytogenetic studies. Blood 94(10 SUPPL 1 PART 1): 699a, Nov 15, 1999

Detection of clonal T-cell receptor gamma-chain gene rearrangements in Reed-Sternberg cells of classic Hodgkin disease. Blood 95(10): 3020-3024, 2000

Mutation of the p53 gene is not a typical feature of Hodgkin and Reed-Sternberg cells in Hodgkin's disease. Blood 94(5): 1755-1760, 1999

Hodgkin's disease with monoclonal and polyclonal populations of Reed-Sternberg cells. New England Journal of Medicine 333(14): 901-906, 1995

Correlations Between Gene Rearrangements and Immunoreactivity of Reed-Sternberg Cells in Paraffin Sections: A Genotypic and Phenotypic Study of 14 Cases of Hodgkin's Disease. Leukemia and Lymphoma 3(4): 267-275, 1991

Production of a monoclonal antibody specific for Hodgkin and Sternberg-Reed cells of Hodgkin's disease and a subset of normal lymphoid cells. Nature 299(5878): 65-67, 1982