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An Aeromonas salmonicida gene required for the establishment of infection in rainbow trout (Oncorhynchus mykiss)



An Aeromonas salmonicida gene required for the establishment of infection in rainbow trout (Oncorhynchus mykiss)



Gene (Amsterdam) 175(1-2): 127-131



The asoB gene of Aeromonas salmonicida is located approximately 9 kb downstream of the structural gene (vapA) for the surface layer (A-layer). The nucleotide sequence of asoB was determined and found to encode a putative polytopic cytoplasmic membrane protein which exhibited homology to a number of bacterial transport proteins. Allele exchange mutagenesis of asoB resulted in a mutant (A449-D) which was avirulent when administered by bath immersion. However, when administered by intraperitoneal injection, A449-D is as lethal as wild type. Characterization of the phenotype of A449-D showed that there were pleiotropic effects on VapA secretion, haemolysis and outer membrane protein composition. Mobilization of cloned asoB on a broad-host-range plasmid into A449-D resulted in the complementation of VapA translocation, haemolytic activity and virulence.

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Accession: 008138126

Download citation: RISBibTeXText

PMID: 8917088

DOI: 10.1016/0378-1119(96)00137-0


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