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An in situ hybridization assay for detection of human cytomegalovirus in the 24th cultures of clinical specimens

An in situ hybridization assay for detection of human cytomegalovirus in the 24th cultures of clinical specimens

Zhonghua Weishengquxue He Mianyixue Zazhi 15(3): 214-216

An in situ hybridization assay for detection of HCMV infection in 24 h culture was described. HCMV nucleic acid probe was directly labelled with horseradish peroxidase (HRP), and the positive signal was shown in purple-brown particles in contrast to the light pink background in the nucleus under a light-microscope when the concentration of proteinase K was 50 to 100 mu-g/ml. The urine specimens of 22 women who had abnormal pregnant history were cultured for 1 day and 7 days, and hybridization in situ with the probe was carried out under the same condition simultaneously. The results showed no significant difference. Thus, the results in situ hybridization assay for 24 h culture were reliable and the method could be a new and fast method for early diagnosis of HCMV infection.

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