An in situ hybridization protocol for detection and identification of planktonic bacteria

Gloeckner, F.O.; Amann, R.; Alfreider, A.; Pernthaler, J.; Psenner, R.; Trebesius, K.; Schleifer, K.H.

Systematic and Applied Microbiology. : 403-406


ISSN/ISBN: 0723-2020
DOI: 10.1016/s0723-2020(96)80069-5
Accession: 008146844

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The fraction of planktonic bacteria in oligo- and mesotrophic lakes that can be classified by in situ hybridization has been significantly increased with an optimized protocol. By the use of white polycarbonate filters, CY3-monolabeled oligonucleotide probes, and high quality fluorescence filter sets between 29 and 64% of the cells visualized by 4',6-diamidino-2-phenylindole (DAPI) can be detected with a bacterial probe or group-specific probes by epifluorescence microscopy without further digital image enhancement.