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An in situ hybridization technique for the study of B19 human parvovirus replication in bone marrow cell cultures


An in situ hybridization technique for the study of B19 human parvovirus replication in bone marrow cell cultures



Journal of Virological Methods 44(2-3): 329-338



ISSN/ISBN: 0166-0934

PMID: 8263124

DOI: 10.1016/0166-0934(93)90067-2

An in situ hybridization technique using digoxigenin labelling was developed to study B19 infection. By using appropriate DNA probes, transcription of structural and non-structural genes was detected in bone marrow cell cultures. Such a simple system is useful to the study of B19-cell interactions in non-permissive cell lines.

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Accession: 008146847

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