An on-line semi-automated solid-phase extraction procedure for high-performance liquid chromatographic determination of lonidamine in serum

Bottalico, C.; Micelli, G.; Guerrieri, A.; Palmisano, F.; Lorusso, V.; de Lena, M.

Journal of Pharmaceutical and Biomedical Analysis 13(11): 1349-1353


ISSN/ISBN: 0731-7085
PMID: 8634352
DOI: 10.1016/0731-7085(95)01542-s
Accession: 008149113

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A semi-automated solid-phase extraction procedure on-line with gradient elution reversed-phase chromatography permits the determination of lonidamine and its metabolite in human serum. The average recovery from serum at the 2.5 mu-g ml-1 level was (92.8 +- 3.4)%. The limit of quantitation for a 100 mu-l sample size was 50 ng ml-1. The within-day (n = 5) and between-day (n = 5) relative standard deviations for lonidamine determination in serum samples spiked at the 2.5 mu-g ml-1 level were 2.7% and 4.5%, respectively.