Section 9
Chapter 8,156

Analysis of interferon-regulated proteins binding the interferon-alpha-stimulated response element

Levy, D.E.

Methods 15(3): 167-174


ISSN/ISBN: 1046-2023
PMID: 9735302
DOI: 10.1006/meth.1998.0621
Accession: 008155150

Interferon mediates its biological effects of antiviral, antiproliferative, and immunomodulatory activities through induction of specific gene expression. Transcription of target genes is regulated through a set of transcription factors that bind to specific cis-acting regulatory sequences in target promoters and enhancers. The activity or abundance of these transcription factors is modulated by interferon treatment. Because of their DNA binding activity, regulatory factors can be recognized and characterized using methods to detect protein-DNA complexes. The availability of antibodies directed against common interferon regulatory proteins coupled with analysis of binding-site specificity provides comprehensive analysis.

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